Development of a Liquid Chromatographic Method for Apomorphine Hydrochloride Quantitation in the Active Pharmaceutical Ingredients and in the Dosage Forms

Apomorphine is currently used as a dopamine agonist to treat advanced Parkinson Disease. In the present study, we developed sensitive, simple, reliable, and robust HPLC method for quality control of apomorphine hydrochloride in bulk drug pharmaceutical formulations. C18 column was separation. Isocratic elution performed using mobile phase A: 50 mM potassium dihydrogen phosphate solution (pH:3 with ortho-phosphoric acid), B: acetonitrile ratio 85:15 (A: B), mobile-phase flow rate kept at 1 mL min-1. Analyses were carried out 272 nm UV detector. A perfect linear relationship between peak-area versus concentration range 10‑100 μg mL-1 observed (r2, 0.9999). It has been found that sensitive (Detection quantification limits determined 1.3 3.8 mL-1, respectively), precise (RSD